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. 2018 Apr 25;8:6519. doi: 10.1038/s41598-018-24628-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Forced expression of FoxO3 reverses miR-9-mediated inhibition of erythroid progenitor cell differentiation and induction of ROS production. (A) qRT-PCR analysis of the expression of FoxO3, and its known down-stream targets in GFP+ fetal liver cells (left panel) and G1ER cells (right panel) transduced with vector or miR-9. (B) Representative histograms of flow cytometric analysis of GFP+ fetal liver cells transduced with Vector, miR-9, FoxO3-3A and FoxO3A/miR-9. (C) Histograms show the average percentage of cells in different stages of erythroid blast differentiation from these transduced fetal liver cells. (D) ROS levels in fetal liver cells transduced with vector, miR-9, FoxO3a and FoxO3a/miR-9. The experiment was performed in triplicate and repeated 2–3 times. *P < 0.05; ***P < 0.005.