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. 2018 Feb 5;37(17):2237–2250. doi: 10.1038/s41388-017-0079-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 3 — Ibrutinib inhibits cell growth in ERBB4^High/BTK^Low cell lines. a Cell proliferation assays were performed in ERBB4^High/BTK^Low and ERBB4^Low/BTK^Low cell lines after ibrutinib treatment (6 days, 1 μM). The number of viable cells is reported as a percentage of the respective control (DMSO treated). b Dose-response curves after ibrutinib treatment (6 days, 1 nM–10 μM). c Protein levels of ERBB4 and downstream proteins (MEK, ERK, and AKT) in ibrutinib-treated H661 cells (0.1–1 μM). d Western blots for ERBB4 and GAPDH in cell lines infected with GFP or shRNA against ERBB4 or scramble. e Protein levels of EGFR and ERBB2 after shRNA infection. f Cell proliferation assay for cells infected with shERBB4, scramble or GFP. g Tumor growth of H661 cell-derived mouse xenografts (n = 10) after daily treatment of ibrutinib (5 mg/kg) and buffer only control for 60 days. The P-value calculated by the two-tailed T-test is shown, and the two-tailed Mann–Whitney U-test P-value was 0.01. (**P < 0.002; ***P < 0.001; ****P < 0.0001)