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. 2018 Apr 25;9(5):488. doi: 10.1038/s41419-018-0537-6

Fig. 6. IDH2 deficiency exacerbates apoptosis after cisplatin administration.

Fig. 6

Idh2‒/‒ mice and wild-type (Idh2+/+) littermates were intraperitoneally injected with either cisplatin (C, 20 mg/kg B.W.) or 0.9% saline (vehicle, V) once. Some mice were treated with Mito-T (M, 0.7 mg/kg B.W.) daily, beginning 7 days before cisplatin injection and continuing until experiments were completed. Kidneys were harvested 3 days after cisplatin injection. a–c Bax and Bcl-2 expression in whole kidney lysates were determined by western blot analysis. GAPDH was used as a loading control. b, c Bax (b) and Bcl-2 (c) band densities were measured using the ImageJ program. d–f Mitochondria and cytosol were fractioned as described in the “Materials and methods” section. Cytochrome c expression was determined in mitochondrial and cytosolic fractions by western blot analysis. e, f Band densities were measured using the ImageJ program and normalized to COX IV and GAPDH. g Cleaved caspase-3 was detected in whole kidney lysates by western blot analysis. h Band density was measured using the ImageJ program and normalized to GAPDH. Results are expressed as means ± SE (n = 3–5 per group). *p < 0.05 vs. respective V; #p < 0.05 vs. respective C; §p < 0.05 vs. V in Idh2+/+; †p < 0.05 vs. C in Idh2+/+