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. 2018 Apr 25;8:6524. doi: 10.1038/s41598-018-24393-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Optical system used for ODT: The optical system is based on Mach-Zehnder interferometric microscopy employing a digital micro mirror device (DMD). Light from a laser coupled in a fiber coupler is split into a sample beam and a reference beam. The sample beam illuminates a sample with incident angles controlled by patterns displayed on the DMD. A beam splitter in front of a camera constructs off-axis geometry for interferometry. This system has a maximum field of view of 50 × 50 µm² with a total magnification of 60×. L1-5 lens; M1-2, mirror; FC, fiber coupler; OL1-2, objective lens; BS, beam splitter; P, polarizer.