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. 2018 Apr 25;8:6543. doi: 10.1038/s41598-018-24884-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcription of TaCOMT-3D in wheat (Triticum aestivum). (A) Expression patterns of TaCOMT-3D in five wheat cultivars with different degrees of resistance to Rhizoctonia cerealis. The expression level of TaCOMT-3D in the Wenmai 6 plants inoculated with R. cerealis for 21 days was set to 1. DI indicates disease index of sharp eyespot. (B) qRT-PCR analysis of R. cerealis Actin (RcActin) gene in CI12633 plants inoculated with R. cerealis at the indicated time. (C) qRT-PCR analysis of TaCOMT-3D induction by R. cerealis inoculation in CI12633 plants. The expression level of TaCOMT-3D at 0 day post inoculation (dpi) with R. cerealis is set to 1. (D) Transcription of TaCOMT-3D in roots, stems, leaves, sheaths, and spikes of Yangmai 16 and CI12633 plants. The transcriptional level of TaCOMT-3D in leaves was set to 1. Statistically significant differences are derived from the results of three independent replications (t-test: **P < 0.01).