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. 2018 Apr 25;8:6543. doi: 10.1038/s41598-018-24884-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing of TaCOMT-3D by barley stripe mosaic virus (BSMV)-induced gene silencing impairs CI12633 resistance to Rhizoctonia cerealis. (A) Scheme of genomic RNAs of BSMV construct and the construct of the recombinant virus expressing the wheat (Triticum aestivum) gene TaCOMT-3D, BSMV:TaCOMT-3D. The orientation of the TaCOMT-3D insert is indicated by dark boxes. (B) Mild chlorotic mosaic symptoms were observed on leaves at 10 days post inoculated (dpi) with BSMV: GFP or BSMV:TaCOMT-3D. (C) RT-PCR analysis of the transcription levels of BSMV coat protein encoding gene CP in the wheat plants infected by BSMV: GFP or BSMV:TaCOMT-3D at 10 dpi with R. cerealis. (D) qRT-PCR analysis of the transcription levels of wheat TaCOMT-3D gene in the wheat plants infected by BSMV: GFP or BSMV:TaCOMT-3D at after infection with R. cerealis. The relative transcript level of TaCOMT-3D in BSMV:TaCOMT-3D-infected (TaCOMT-3D-silencing) wheat CI12633 plants is relative to that in BSMV: GFP-infected (control) plants (set to 1). Significant differences were analyzed based on three replications (t-test: **P < 0.01). Error bars indicate standard deviation. (E) Sharp eyespot symptoms of the control and TaCOMT-3D-silencing CI12633 plants at 45 dpi with R. cerealis. IT indicates the infection type of wheat plant response to R. cerealis.