Fig. 6.

Recruitment of CCR-6-expressing B cells alters the TME to promote CAC. a Body weight loss in % of NCD-fed WT (n = 17) and JHT (n = 14) mice at day 0 to day 13 of the 1.5% AOM/DSS protocol. b, c qPCR analysis of indicated gene expression in colitic colons of 10-week-old NCD-fed WT and JHT (n = 3–9) mice at day 13 of the 1.5% AOM/DSS protocol, results are presented relative to non-colitic WT colons at day 0. d Representative endoscopic pictures of 13-week-old NCD-fed WT and JHT mice of the 1.5% AOM/DSS protocol. e H&E staining of colons from NCD-fed 17-week-old WT and JHT mice at day 62 of the 1.5% AOM/DSS protocol. f Tumour number of 17-week-old NCD-fed WT (n = 11) and JHT (n = 7) mice counted at day 62 of the 1.5% AOM/DSS protocol. g, h qPCR analysis of indicated gene expression in colons of 17-week-old NCD-fed WT (n = 7) and JHT (n = 9) mice at day 62 of the 1.5% AOM/DSS protocol, results are presented relative to non-colitic WT colons at day 0. i Representative immunofluorescent stainings of CD3 (green)-expressing T cells and B220 (red)-expressing B cell in colons of 17-week-old NCD-fed WT and JHT mice at day 62 of the 1.5% AOM/DSS protocol. j, k qPCR analysis of indicated gene expression in colons of 17-week-old NCD-fed WT (n = 7) and JHT (n = 9) mice at day 62 of the 1.5% AOM/DSS protocol, results are presented relative to non-colitic WT colons at day 0. AOM, azoxymethane; DSS, dextran sodium sulphate; NCD, normal chow diet; CAC, colitis-associated colorectal cancer; TME, tumour microenviroment; JHT, targeted deletion of the JH locus. Data are represented as mean + SEM or centre line: median; box limits: 1st and 3rd quartiles; whisker: maximum to minimum, *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001 two-tailed unpaired Student’s t-test f or two-way ANOVA followed by Fisher LSD a, b, c, g, h, j, k. Scale bar, 50 μm e, 200 μm i