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. 2018 Apr 25;8:6535. doi: 10.1038/s41598-018-24964-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential expression of the two hlyCABD operons present in the J96 strain. (a) Percentage of identity at the level of the nucleotide sequence between the operon hly_I and hly_II of J96 and 536. n.d.: not determined. (b) Hemolytic phenotype of J96, JFV16 (J96ΔII) and JFV21 (J96ΔI) strains on Columbia Blood agar plates. (c) Coomassie blue stained SDS-PAGE (10%) of secreted protein extracts from cultures grown in LB₀ at 37 °C up to late-log phase (OD_600 nm of 1.0) of the indicated strains. Lane M: molecular mass markers (size in kDa indicated along the left side). The band corresponding to α-hemolysin (HlyA) is indicated with an arrowhead. Full-length gel image is shown in Fig. S6.