Table 1.

Physicochemical characterization of the selected nanocapsules (NCs).

Formulation	Abbreviation	Size (nm)	PDI	Zeta potential (mV)	rHBAg association (%)
Nanoemulsion	NE	264 ± 10	0.2	–23 ± 2	–
Chitosan nanocapsules	CS NCs	456 ± 2	0.3	+47 ± 2	–
Protamine nanocapsules	PR NCs	274 ± 4	0.2	+27 ± 4	–
rHBsAg-loaded protamine nanocapsules	PR NCs-rHBsAg	358 ± 20	0.3	+5 ± 4	74 ± 5
Polyarginine nanocapsules	PARG NCs	251 ± 2	0.1	+30 ± 2	–
rHBsAg-loaded polyarginine nanocapsules	PARG NCs-rHBsAg	320 ± 8	0.3	+15 ± 1	70 ± 7

PDI, polydispersity index; HBsAg, hepatitis B surface antigen.