Fig. 4. Effects of JZL195 on brain endocannabinoid levels, locomotor activity, anxiety-like behavior in the open-field test and body temperature.

a Dual fatty acid amide hydrolase and monoacylglycerol lipase inhibition effects on brain N-arachidonylethanolamine (AEA), 2-arachidonoylglycerol (2-AG), arachidonic acid (AA) and oleoylethanolamine (OEA) levels. b Effects of JZL195 on % center time, % center distance, total distance, average velocity and number of fecal boli in the open-field test. c JZL195 treatment dose-dependently lowered body temperature. d Brain AEA and e 2-AG correlations with various behavioral parameters. Significant F and P-values from one-way analysis of variance noted above bar graphs; *P < 0.05, ***P < 0.001, ****P < 0.0001 vs vehicle group by Holm–Sidak post hoc multiple comparisons test in bar graphs. Linear regression (solid line) with 95% confidence intervals (dashed lines) shown in figures. Data are presented as means ± SEM