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. 2018 Apr 25;8:6533. doi: 10.1038/s41598-018-24908-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell types used in this study. a, b: N2A cells before (a) and three days after serum deprivation (b). (c,d) Primary mouse cortical neurons, 7 (c) and 14 (d) days after start of culture. Mature cells formed dense clusters that did not allow for single cell stimulation. Therefore, neurons were used at the earlier time point. Scale bar corresponds to all panels.