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. 2018 Apr 25;8:6533. doi: 10.1038/s41598-018-24908-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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∆F/F0 and ttp values from N2A (a,b) and MCN (c,d) versus radiant exposure. (a,b) Mean values and standard deviations were calculated for each experiment with n = 10 cells (circles + error bars). Black triangles are averages of the respective means per radiant exposure and grey bars are the associated 95%-confidence intervals. While there is no visible trend in ∆F/F₀ values, time to peak decreased with increasing radiant exposure. (c,d) Mean values and standard deviations were calculated from one experiment with n = 10 cells. Larger values for radiant exposure tended to yield larger peak fluorescence values, but also resulted in larger variation. Time to peak values do not show a clear trend. MCN were not activated at 17 mJ/cm². (e) The proportion of PI-positive N2A cells (pooled) increased with radiant exposure.