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. 2018 Apr 25;8:6533. doi: 10.1038/s41598-018-24908-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence signals and peak fluorescence values for the green-emitting (oxidized) form of BODIPY 581/591 after laser stimulation. Control experiments were performed without gold nanoparticles. (a) Representative traces of fluorescence increase over time in N2A cells after laser stimulation with and without AuNP. In the presence of nanoparticles, the dye’s fluorescence increase was both faster and more pronounced. (b,c) In N2A cells, ∆F/F₀ values increased in the presence of AuNP. No distinction can be made by power. PBSC might however strengthen the oxidizing effect. Lipidperoxidation in MCN was only assessed at 51 mJ/cm². In these cells, the oxidation by the laser alone appears to be of a similar magnitude as in the presence of AuNP.