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. 2018 Apr 19;9:242. doi: 10.3389/fphar.2018.00242

FIGURE 2.

FIGURE 2

Manipulation of the P2Y12 receptor alters lysosomal pH in ARPE-19 cells. (A) The lysosomal pH of ARPE-19 cells was elevated by P2Y12 receptor agonist methylthio ADP (MeS-ADP, 10 μM). ∗p = 0.002, n = 9–10. (B) Ticagrelor (20 μM) lowered the lysosomal pH in cells treated with MeS-ADP (∗p = 0.006, n = 13). (C) The P2Y12 receptor antagonist AR-C66931 (ARC, 10 μM) also reduced lysosomal pH in ARPE-19 cells treated with MeS-ADP (∗p = 0.033, n = 10). Data are expressed as the ratio of fluorescence excited at 340 vs. 380 nm in cells loaded with LysoSensor Yellow/Blue DNS 160; this is indicative of lysosomal pH, although absolute ratios varied with preparation; n represents the number of wells in a single trial with figures representative of 2–4 trials.