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. 2018 Apr 19;9:820. doi: 10.3389/fimmu.2018.00820

Figure 3.

Figure 3

GSC718 and GSC839 augment B cell proliferation induced by anti-CD40 and TLR ligands in vitro. Spleen B cells obtained from wild type (WT) (A–H), CD22−/− (A–D), and ST6GalI−/− (E–H) C57BL/6 mice were stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) and cultured for 72 h in the presence of indicated concentrations of anti-CD40 (A–H), CpG oligo (A–H), or LPS (E–H) with or without 50 µM GSC718 (A,B,E,F) or GSC839 (C,D,G,H). Cells were analyzed by FCM and percentages of proliferated cells are indicated (A,C,E,G). Data are representative of at least three experiments. Mean ± SD (n = 3) is shown (B,D,F,H). Data were analyzed by unpaired t-test. **P < 0.01, ***P < 0.001, NS, not significant.