Table 3.

Enzyme kinetic parameters for glucan, xylan, and mannan mono- and oligo-saccharide inhibition of TtCBH6 and TtCBH7 using MUG2 as a substrate.

		TtCBH7			TtCBH6
		Vmax (μM/min)	Km/Kmapp (μM)	Ki (mM)	Vmax (μM/min)	Km/Kmapp (μM)
MUG2		41 × 10∧3	0.90		33 × 10∧3	0.42
Cellobiose	5 mM	25 × 10∧3	4.04	1.43	33 × 10∧3	0.40
	10 mM	35 × 10∧3	6.54	1.59	34 × 10∧3	0.45
Glucose	5 mM	39 × 10∧3	1.00		31 × 10∧3	0.39
	10 mM	54 × 10∧3	1.58	13.23	33 × 10∧3	0.41
Xylotriose	5 mM	32 × 10∧3	0.77		32 × 10∧3	0.42
	10 mM	36 × 10∧3	0.95		28 × 10∧3	0.46
Xylobiose	5 mM	33 × 10∧3	0.81		30 × 10∧3	0.44
	10 mM	40 × 10∧3	1.17	33.3	32 × 10∧3	0.41
Mannotriose	5 mM	36 × 10∧3	0.88		30 × 10∧3	0.41
	10 mM	39 × 10∧3	0.98		34 × 10∧3	0.44
Mannobiose	5 mM	36 × 10∧3	0.91		31 × 10∧3	0.38
	10 mM	38 × 10∧3	1.04		29 × 10∧3	0.35
Xylosyl-Cellobiose	5 mM	37 × 10∧3	0.99		30 × 10∧3	0.43
	10 mM	39 × 10∧3	1.01		33 × 10∧3	0.48

There was no inhibition observed for TtCBH6 upon the addition of 5 and 10 mM concentration of different inhibitors. K_m-value was calculated from the reaction in the absence of inhibitors.