Table 4.
Preliminary experiments with various CBHI:EG5 ratios.
| Birch | Spruce | |||
|---|---|---|---|---|
| CBH7:EG5 ratio | Cellobiose mg/mL | Cellobiose yield (%) (mg/g glucan) | Cellobiose mg/mL | Cellobiose yield (%) (mg/g glucan) |
| 1:100 | 2.87 ± 0.12 | 19.0 (190) | 1.96 ± 0.04 | 13.2 (132) |
| 40:60 | 5.43 ± 0.89 | 35.9 (359) | 4.19 ± 0.31 | 28.2 (282) |
| 50:50 | 5.74 ± 0.67 | 38.0 (380) | 4.57 ± 0.23 | 30.7 (307) |
| 60:40 | 5.78 ± 0.51 | 38.3 (383) | 4.62 ± 0.61 | 31.1 (311) |
| 70:30 | 5.48 ± 0.55 | 36.3 (363) | 4.65 ± 0.50 | 31.3 (313) |
| 80:20 | 5.08 ± 0.46 | 33.6 (336) | 4.51 ± 0.11 | 30.3 (303) |
| 90:10 | 4.64 ± 0.21 | 30.7 (307) | 4.41 ± 0.14 | 29.7 (297) |
| 100:1 | 3.57 ± 0.39 | 23.7 (237) | 3.82 ± 0.22 | 25.7 (257) |
The reactions were performed with 25 mg total enzyme/g substrate and incubated for 48 h. The results are expressed as a percentage of the conversion of the total glucan content of the substrates. Bold values indicate the enzyme combinations giving the maximal hydrolysis yields.