Table 5.

Model prediction values and experimental results of the optimized mixtures and final % cellobiose yields, after 24 and 48 h of reaction for #1 and #2 experimental design.

#1	Substrate	Optimal enzyme proportions (%)				Cellobiose yield (%)
		CBH7	CBH6	EG5	EG7	Mod. Pr.	Exp. data
24 h	Spruce	66.6	5.0	23.3	5.0	22.7	22.3 ± 1.7
	Birch	58.5	6.5	30.0	5.0	26.4	27.1 ± 2.3
48 h	Spruce	50.0	12.0	27.0	11.0	37.7	36.2 ± 0.5
	Birch	50.0	5.0	30.0	15.0	43.8	40.2 ± 1.2
#2	Substrate	Optimal enzyme proportions (%)				Cellobiose yield (%)
		CBH7	EG5	EG9	EG6	Mod. Pr.	Exp. data
48 h	Spruce	64.9	21.6	4.3	9.2	42.5	40.0 ± 2.1
	Birch	69.3	20.0	9.7	1.0	41.6	42.7 ± 1.1

In experimental design #2, CBH6 and EG7 were kept constant and equal with those values that gave the maximal cellobiose yield in design #1. The % cellobiose yields have been calculated on the basis of total glucan content of initial substrates.