Table 6.

Experimental values of % cellobiose yields after 24 and 48 h of reaction, using the optimized ternary mixtures identified by #1 and #2 designs, after replacement of commercial CBH6 and CBH7 with TtCBH6 and TtCBH7 that were produced and characterized in the present study.

#1	Substrate	Optimal enzyme proportions (%)				Cellobiose yield (%) (mg/g glucan)
		TtCBH7	TtCBH6	EG5	EG7
24 h	Spruce	66.6	5.0	23.3	5.0	18.4 ± 2.7 (184)
	Birch	58.5	6.5	30.0	5.0	28.1 ± 0.3 (281)
48 h	Spruce	50.0	12.0	27.0	11.0	32.0 ± 1.1 (320)
	Birch	50.0	5.0	30.0	15.0	35.2 ± 1.8 (352)
#2	Substrate	Optimal enzyme proportions (%)				Cellobiose yield (%) (mg/g glucan)
		TtCBH7	EG5	EG9	EG6
48 h	Spruce	64.9	21.6	4.3	9.2	36.0 ± 0.9 (360)
	Birch	69.3	20.0	9.7	1.0	35.2 ± 2.1 (352)

The % cellobiose yields have been calculated on the basis of total glucan content of initial substrates.