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. 2018 Apr 25;3(2):e00023-18. doi: 10.1128/mSphere.00023-18

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Copyright © 2018 Reuwsaat et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Recombinant Krp1 is antigenic. (A) Western blot. Purified recombinant truncated Krp1 (rKrp1t) expressed in E. coli was serially diluted (1:2) and probed with pooled sera from cryptococcal patients (top panel). SDS-PAGE mirror gel (bottom panel). (B) ELISA. One microgram of purified rKrp1t was probed with sera from patients with cryptococcosis and candidiasis. Results were analyzed by unpaired t tests. OD readings to determine IgG and IgM are shown. Values that are significantly different (P < 0.001) by t test are indicated by three asterisks.