Figure 1.

crhR transcript accumulation in response to illumination and/or exogenous carbon sources. A DNA probe internal to the crhR coding region was used to detect the crhR transcript in total RNA (6 μg) extracted from mid-log phase Synechocystis cultures grown photoautotrophically, or with addition of Glc (5 mm) or 3-OMG (5 mm), and treated as indicated. The 1.6-kb crhR transcript is shown. W, White light; D, darkness. Relative RNase P transcript levels are shown below each lane as a control for RNA loading.