Table IV.

ATP sulfurylase in high-speed supernatant and pellet fractions from isolated cystosol

Fraction	Volume	Protein Concentration	Protein	SA	Total Activity	Recovery of Total Cytosol
	mL	mg/mL	mg	nmole min−1 mg−1	nmol min−1	%
Total cytosol	1.50	0.6	0.90	45	41	100
S140	1.49	0.6	0.89	56	50	122
P140	0.08	0.2	0.02	0	0	0

The cytosolic fraction isolated from 47-d-old plants was subjected to ultracentrifugation and 140,000g. The activity of cytosolic ATP sulfurylase in the soluble and particulate fractions was measured. Each value represents the mean of two independent measurements. SA, Specific activity.