Figure 2.

Innate-like T cell markers on CD161-expressing T cells. (A) Flow cytometric gating of TCRγδ⁺ cells in the CD4⁺ and CD8⁺ T cell compartment of a young and old subject. (B) Percentages of TCRγδ⁺ cells within the CD161-defined CD4⁺ and CD8⁺ T cell subsets of 7 young [of which 4 were cytomegalovirus (CMV) seropositive] and 16 old (of which 8 were CMV seropositive) subjects. (C) Flow cytometric gating of TCR-Vα24-Jα18⁺TCR-Vβ11⁺ cells in the CD4⁺ and CD8⁺ T cell compartment of a young and old subject. (D) Percentages of TCR-Vα24-Jα18⁺ TCR-Vβ11⁺ cells within the CD161-defined CD4⁺ and CD8⁺ T cell subsets of nine young (of which four were CMV seropositive) and nine old (of which four were CMV seropositive) subjects. (E) Flow cytometric gating of TCR-Vα7.2⁺ cells in the CD4⁺ and CD8⁺ T cell compartment of a young and old subject. (F) Percentages of TCR-Vα7.2⁺ cells within the CD161-defined CD4⁺ and CD8⁺ T cell subsets of 10 young (of which 5 were CMV seropositive) and 10 old (of which 5 were CMV seropositive) subjects. Graphs in which CMV-seropositive young and old subjects are highlighted in the figures are shown in Figure S2 in Supplementary Material.