Figure 5.

Natural killer markers on CD161-expressing T cells. (A) Flow cytometric gating of 2B4⁺ cells within the CD4⁺ and CD8⁺ T cell compartment of a young and old subject. (B) Percentages of 2B4⁺ cells within the CD161-defined CD4⁺ and CD8⁺ T cell subsets of 8 young [of which 4 were cytomegalovirus (CMV) seropositive] and 15 old (of which 7 were CMV seropositive) subjects. (C) Flow cytometric gating of DNAX accessory molecule-1 (DNAM-1)⁺ cells within the CD4⁺ and CD8⁺ T cell compartment of a young and old subject. (D) Percentages of DNAM-1⁺ cells within the CD161-defined CD4⁺ and CD8⁺ T cell subsets of the same subjects as shown in (B). (E) Flow cytometric gating of NKG2D⁺ cells within the CD4⁺ and CD8⁺ T cell compartment of a young and old subject. (F) Percentages of NKG2D⁺ cells within the CD161-defined CD4⁺ and CD8⁺ T cell subsets of 11 young (of which 6 were CMV seropositive) and 14 old (of which 8 were CMV seropositive) subjects. (G) Flow cytometric gating of KLRG1⁺ cells within the CD4⁺ and CD8⁺ T cell compartment of a young and old subject. (H) Percentages of KLRG1⁺ cells within the CD161-defined CD4⁺ and CD8⁺ T cell subsets of the same subjects as shown in (F). Statistical significance by Mann–Whitney U-test is shown as *p < 0.05 or **p < 0.01. Graphs in which CMV-seropositive young and old subjects are highlighted in the figures are shown in Figure S5 in Supplementary Material.