Figure 1.

Production of superoxide anion (O₂⁻) in response to hypo-osmotic shock. A, Inhibition by DPI of O₂⁻ production. The cells were pretreated with or without 30 μm DPI for 30 min, then stimulated by hypo-osmotic shock. Control cells were not subjected to the stimulus. Dimethyl sulfoxide alone was added to the solvent control of DPI, which was also stimulated by hypo-osmotic shock. B, Accumulation of O₂⁻ in the presence of a superoxide dismutase (SOD) inhibitor diethyldithiocarbamate (DDC). The cells were pretreated with or without 1 mm DDC for 10 min, then stimulated by hypo-osmotic shock. Control cells were not subjected to the stimulus. Results shown are representatives from three (A) and two (B) similar independent experiments.