Figure 2.

Identification of a Rac/Rop-related protein in soybean cells by immunoblotting and [γ-³⁵S] GTP-binding assay. Crude extracts from suspension-cultured soybean cells were separated by 15% SDS-PAGE, transferred to nitrocellulose membrane, and probed with antibodies raised against Rac1(C189S) (lane 1), Rop (lane 2), Rac1 (lane 3), and Rac2 (lane 4). Nitrocellulose membrane prepared in the same manner was also used in [γ-³⁵S] GTP-binding assay, the result of which was visualized by autoradiography (lane 5). Representatives from two independent experiments with similar results are shown.