Figure 4.

Altered rates of H₂O₂ production by mutant Rac1-transformed soybean cells. Oxidative burst assay of suspension-cultured soybean cells transformed with RacN17 (b), RacV12 together with GUS (d), or GUS alone (c) in response to hypo-osmotic shock (A), OGA (B), and harpin (C). a, A control showing RacV12-transformed cells in the absence of any stimuli. Controls using all other transformants showed similar response. The inset of A shows western hybridization using anti-myc antibody to probe total protein extracted from soybean cells transformed with myc-tagged RacV12 (lane 1), RacN17 (lane 2), and the GUS gene without an epitope tag (lane 3). Results shown are representatives from four (osmotic shock), five (OGA), and two (harpin) similar independent experiments. Each experiment consisted of three replicates each for each sample type.