Figure 6.

Immunization with Replicative Defective Adenoviral Vectors Induces Three Subsets Based on CX3CR1 Expression in CD8⁺ Memory T Cells in Humans

CX3CR1 expression was assessed in adenoviral vaccine-derived CD8⁺ T cells in blood obtained from 9 volunteers. Blood was taken at peak response after ChAd3-NSmut prime (trial weeks [TW] 2–4, prime, n = 9), peak response after MVA-NSmut boost (TW9, boost, n = 5), and at end of study (TW16–TW32, EOS, n = 9). Vaccine-derived CD8⁺ T cells were identified by staining with an HLA-A2 pentamer containing an HCV NS3 epitope (A2-NS3 pentamer).

(A) Composite FACS plots of A2-NS3 pentamer staining (left) of live CD3⁺ lymphocytes and CX3CR1 subsets of A2-NS3 pentamer⁺ CD8⁺ T cells. Mean A2-NS3 pentamer⁺ CD8⁺ T cell frequency and mean CX3CR1 subset frequency are indicated.

(B) Pie chart showing mean CD8⁺ T cell subset distribution of A2-NS3 pentamer⁺ CD8⁺ T cells.

(C) CX3CR1 subset frequency of A2-NS3 pentamer⁺ CD8⁺ T cells.

(D and E) CX3CR1 subset expression of cell surface markers (CD27 and CD127) and transcription factors (T-bet and Eomes) in A2-NS3 pentamer⁺ CD8⁺ T cells.

(D) Composite FACS plots (L to R) of CD27, CD127, T-bet, and Eomes expression on A2-NS3 pentamer⁺ CD8⁺ T cells. Mean expression for each CX3CR1 subset is indicated.

(E) Individual values and mean CX3CR1 subset expression (L to R) of CD27, CD127, T-bet, and Eomes in A2-NS3 pentamer⁺ CD8⁺ T cells.

SSDs were determined by one-way ANOVA (C) or two-way ANOVA (E) and corrected for multiple comparisons (Holm-Sidak). ^∗p = 0.05 to 0.011, ^∗∗p = 0.01 to 0.001, ^∗∗∗p < 0.001. Data are compiled from a single time course experiment. See also Figure S5.