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Japanese Journal of Cancer Research : Gann logoLink to Japanese Journal of Cancer Research : Gann
. 1990 Oct;81(10):1012–1020. doi: 10.1111/j.1349-7006.1990.tb03339.x

Induction of Killer Cells from Lymphocytes in Pleural Effusion of Advanced Lung Cancer Patients

Yuji Inoue 1, Noriharu Shijubo 1,✉, Toshimitsu Uede 2
PMCID: PMC5917975  PMID: 2172193

Abstract

We analyzed the phenotype and cytotoxic ability of pleural exudative lymphocytes (PLEL) which were obtained from 18 advanced lung cancer patients. Freshly isolated PLEL were mainly CD4(+) T cells and had weak natural killer, autologous tumor killing and lymphokine‐activated killer activities. After cultivation of PLEL with interleukin‐2, cytotoxicity of PLEL against autologous tumor cells was increased at 2 weeks, but it was remarkably reduced at 4 weeks. When PLEL were stimulated by mitomycin C‐treated autologous tumor cells during culture, autologous tumor killing activity of PLEL was significantly enhanced even after 4 weeks of cultivation. Cold target inhibition analysis and binding inhibition assays using monoclonal antibodies indicated that autologous tumor stimulation could induce major histocompatibility complex class I restricted cytotoxic T lymphocytes specific for autologous tumor cells in some cases.

Keywords: Lung cancer, Pleural exudative lymphocytes, Cytotoxic T lymphocytes, Autologous tumor stimulation

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