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Analysis of Transduced Murine Lin− Cells Maintained in Bulk or Clonogenic Culture
| VCN (Bulk) | IL2RG mRNA (Bulk) | GFP+ Cells (%) | LV+ CFCs (%) | |
|---|---|---|---|---|
| WT + GFP | 3.5 ± 0.4 | 1 | 80.5 ± 0.7 | 88 |
| KO + GFP | 2.1 ± 0.5 | – | 64 ± 9 | 78 |
| KO + IL2RG 1 hit | 2.5 ± 1.1 | 0.46 ± 0.22 | – | 78 |
| KO + IL2RG 2 hits | 4.5 ± 1.0 | 1.17 ± 0.47 | – | 92 |
