Table 2.

Aptamer Distribution Parameters from Fitting with Gamma Variate Summation Model

Formulation	k1 (Molecules/mg Tissue)	k2 (Molecules/mg Tissue)	a1 (Dimensionless)	a2 (Dimensionless)	b1 (min)	b2 (min)	A(∞) (min-Molecules/mg Tissue)
Brain
3064-4WJ	1.9 + 1.6 × 107	9.5 + 2.0 × 105	0.70 ± 0.25	3.1 ± 2.4	8.6 ± 1.4	380 ± 250	6.9 ± 1.4 × 108
3064-4WJ-LNA	1.1 ± 0.4 × 105	8.5 ± 0.9 × 104	0.24 ± 0.05	6.9 ± 0.4	56.0 ± 8.4	1,700 ± 100	2.5 ± 0.2 × 108
3064-B	4.6 ± 1.1 × 107	1.6 ± 0.3 × 105	1.20 ± 0.97	13.0 ± 2.8	11.0 ± 4.5	2,000 ± 600	1.1 ± 0.2 × 109
3064-BS	1.1 ± 0.4 × 107	4.4 ± 0.9 × 104	0.58 ± 0.21	37.0 ± 8.0	7.6 ± 0.9	8,400 ± 2,600	7.4 ± 2.8 × 108
Spinal Cord
3064-4WJ	2.0 ± 0.9 × 107	1.6 ± 0.3 × 105	0.34 ± 0.15	3.3 ± 0.6	15.0 ± 4.9	710 ± 80	8.8 ± 2.2 × 109
3064-4WJ-LNA	4.2 ± 3.1 × 105	1.9 ± 0.4 × 104	0.22 ± 0.17	36.0 ± 9.4	23.0 ± 9.2	8,200 ± 2,700	4.6 ± 1.6 × 108
3064-B	7.4 ± 3.1 × 107	6.2 ± 3.2 × 104	1.20 ± 1.54	6.0 ± 3.6	15.0 ± 4.9	1,000 ± 300	2.4 ± 0.8 × 109
3064-BS	1.6 ± 0.4 × 107	5.9 ± 0.5 × 104	2.90 ± 2.50	4.9 ± 0.2	20.0 ± 3.8	1,200 ± 50	5.8 ± 1.0 × 108
Serum
3064-4WJ	2.6 ± 0.7 × 107	1.1 ± 0.5 × 105	0.51 ± 0.10	80.0 ± 21.7	58.0 ± 14.4	840 ± 210	2.9 ± 0.9 × 109
3064-4WJ-LNA	2.6 ± 1.0 × 106	4.8 ± 4.6 × 104	0.30 ± 0.12	25.0 ± 18.1	30.0 ± 10.3	4,600 ± 1,100	7.0 ± 1.6 × 108
3064-B	1.2 ± 0.3 × 108	3.1 + 0.4 × 104	0.32 ± 0.06	5.9 ± 1.7	6.1 ± 0.5	1,300 ± 300	2.7 ± 0.6 × 109
3064-BS	1.9 ± 0.4 × 106	1.1 ± 0.2 × 105	0.70 ± 0.24	3.1 ± 0.6	10.0 ± 1.2	640 ± 20	1.2 ± 0.1 × 108

A(∞) is area under the curve.

Fitting is to the following model:

$$C\left(t\right)=k_1{\left(\frac{\ln 2}{b_1}t\right)}^{\frac{\ln 2}{a_1}}{e}^{-\frac{\ln 2}{b_1}t}+k_2{\left(\frac{\ln 2}{b_2}t\right)}^{\frac{\ln 2}{a_2}}{e}^{-\frac{\ln 2}{b_2}t}.$$