Figure 5.

RAS Controls the Switch in CADHERIN Expression in the Transition to the Primed State

(A and B) mESCs grown in 2i/LIF were switched to KSR/bFGF, grown for 10 passages, and subjected to western blot analysis (A) and immunofluorescent staining (B) of E-CADHERIN (E-CAD) or N-CADHERIN (N-CAD).

(C–E) mESCs were grown in 2i/LIF conditions and transfected with a plasmid encoding for GFP fused to H-Ras (GFP-H-RAS) or GFP alone. Immunostaining (C and D) or western blot analysis (E) of the indicated protein is shown.

(F–H) mESCs were grown in KSR/bFGF in the presence of RASi (75 μM) or Veh for 48 hr and then subjected to immunostaining or western blot analysis of the indicated CADHERIN.

(B, E, and H) Values represent densitometry analysis of three independent experiments. Scale bar, 50 μm.