Figure 2.

Canonical Signaling Pathways Are Differentially Activated in Dispersed and Myofiber-Associated Myoblasts

(A) Genes differentially expressed between 48 and 72 hr post-isolation in dispersed (Dis) and myofiber-associated (Mf-A) myoblasts were functionally mapped to all canonical signaling pathways listed by IPA. For each signaling pathway, an enrichment p value and a Z score of activation were calculated and the pathways with enrichment p value < 0.01 (–log[p value] > 1.3) are plotted as heatmaps, were orange represents a positive Z score (= activation), blue is a negative Z score (deactivation), and white is Z = 0. Canonical signaling pathways that were not enriched enough to show a Z score were excluded. Canonical signaling pathways that change in opposite direction in dispersed and myofiber-associated myoblasts are highlighted by a green box.

(B–D) Heatmaps obtained as in (A) for canonical signaling pathways mapping to the IPA categories: Cell Cycle (B), Cell Growth and Proliferation (C), and Growth Factor Signaling (D).

(E) Heatmap distribution of the comparative ratio (CR) = ([72 hr/48 hr]_dispersed/[72 hr/48 hr]_{myofiber-associated}) for gene families that are involved in myogenesis (manual annotation, see Experimental Procedures section).