Skip to main content
. 2018 Mar 1;10(3):890–904. doi: 10.1016/j.stemcr.2018.01.011

Figure 6.

Figure 6

Inhibition of NOTCH Signaling Counteracts Meso-Endothelial Bipotent and Endothelial Progenitor Cell Growth

(A) Placental cells were sorted and cultured in the presence of the γ-secretase inhibitor (DAPT, 50 μM) or DMSO (control group) in endothelial growth medium (EGM2).

(B) Neither CD31Lo nor CD31Int population grew in the presence of DAPT, while culture in DMSO resulted in meso-endothelial bipotent cells (CD31Lo) and EPC (CD31Int) colonies in passage 1.

(C and D) Placental cells were sorted and cultured in the culture slides for 2 weeks and the primary colonies were stained for CD144 and HEY 1 (C) or CD144 and NOTCH1 (D) antibodies.

Scale bars, 100 μm (A and B) and 20 μm (C and D).