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. 2018 Mar 1;10(3):890–904. doi: 10.1016/j.stemcr.2018.01.011

Figure 7.

Figure 7

Differentiation of Meso-Endothelial Bipotent Cells to Mesenchymal Lineage Is Not Mediated by the TGFβ Pathway

(A) On sorting, CD45−CD34+ subpopulations were cultured with SB431542 (TGFβ inhibitor, 10 μM) or DMSO (control group).

(B) Culture in the presence of SB431542 resulted in bipotential (CD31Lo) and EPC (CD31Int) colonies in passage 1.

(C) Addition of SB431542 did not inhibit the growth of CD31Neg, Low or Int populations in passage 1.

(D) TGFβ pathway inhibition increased the level of colony-formation capacity in CD31Lo population compared with the control group (ns, not significant; result from three independent placentas).

(E) qRT-PCR analysis confirmed that expression of endothelial genes (PECAM-1, CD34, and CD144) was not affected in the presence of SB431542 compared with the CD31Int population.

(F) Placental sections were stained for CD31 and SNAIL SLUG markers.

(G) Placental cells were sorted and cultured on the culture slides for 2 weeks, then primary colonies were stained for CD31 and SNAIL SLUG antibodies. Scale bars, 100 μm (B, C, and F) and 20 μm (G).