Figure 5.

T-UCstem1 Silencing Promotes ESC Neural Differentiation

(A) Representative photomicrographs of Control (NT) and T-UCstem1 KD (KD) ESCs differentiated in neurons. Scale bar, 100 μm.

(B) Time course expression profiles of neural (Sox1, Nestin, and βIII-tubulin) and glial (GFAP) markers in Control (NT) and two independent T-UCstem1 KD clones. RNA expression level was normalized to Gapdh expression. Data are mean ± SEM (n = 3 independent experiments); ^∗∗p < 0.005, ^∗∗∗p < 0.001.

(C) Representative pictures of OCT4/NESTIN, OCT4/βIII-TUBULIN, and GFAP/βIII-TUBULIN double immunostaining in Control (NT) and KD ESC neural differentiation at the indicated time points. Nuclei were stained with DAPI. Scale bars, 75 μm.

(D) FACS-based quantification of NESTIN (day 4), βIII-TUBULIN (day 12), and GFAP (day 12) positive cells in Control (NT) and KD ESC neural differentiation. Data are mean ± SEM (n = 3 independent experiments). See also Figure S5.