Mitochondrial Gene Expression in iPSCs and iPSC-CMs
(A) mtDNA copy number was performed by qPCR.
(B) qPCR analysis of 16S rRNA from HCM-iPSC-CMs compared with Con-iPSC-CMs.
(C) Western blotting and quantification analysis of mitochondrial large ribosomal subunit 16S rRNA binding proteins (MRPL19 and MRPL23). VDAC is shown as a loading control.
(D) Western blotting and quantification analysis of respiratory electron transport chain complex subunits, including MT-ND5, MT-CYB, MT-COX2, and MT-ATP8. VDAC is shown as a loading control.
Data are represented as mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001.