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. 2000 Oct;124(2):781–794. doi: 10.1104/pp.124.2.781

Figure 2.

Figure 2

Expression of IFS mRNA, protein, and enzyme activity in tobacco leaves. A, Northern blot of RNA from IFS transgenic tobacco plants. Samples contain approximately 20 μg of total RNA. Lane 1, Wild-type tobacco leaves; lane 2, transgenic number 16 petals; lane 3, transgenic number 16 leaves; lane 4, transgenic number 3 petals; lane 5, transgenic number 3 leaves. The band that hybridizes to the IFS probe is labeled. The same lanes with ethidium bromide-stained rRNA control bands are shown below. B, Western blot of microsomal proteins from IFS transgenic tobacco leaves. Lane 1, Wild-type tobacco leaves; lane 2, transgenic number 3 leaves; lane 3, transgenic number 17 leaves. The band that reacts with the IFS antiserum is labeled. C, Chromatograms at 260 nm of products of the tobacco leaf microsome IFS assay samples from wild type (top) and IFS transformant number 3 (bottom). The naringenin and genistein peaks are labeled.