Figure 3.

Low-Phagocytic RPE Cells Possess a Highly Abnormal F-Actin Cytoskeleton Characterized by Lack of Lateral Circumferential F-Actin, Less Apical F-Actin, and Abundant Stress Fibers

(A and B) Images show acquired representative maximal projections of F-actin (green) for high-phagocytic RPE line #1 (A) or low-phagocytic RPE line #5 (B) as indicated using identical imaging settings.

(C) Bar graph shows percentage of cells with complete lateral circumferential F-actin and no stress fibers in each of the eight RPE lines as indicated. Error bars show mean ± SEM, n = 3 independent experiments for each line. Asterisks indicate significant differences between cell lines as indicated by lines by one-way ANOVA. ^∗p < 0.0001.

(D) Representative images obtained using identical imaging settings show F-actin (green) and cell nuclei (red) co-stains in separate individual x-y confocal sections representative for apical, central, and basal aspects of cells and an x-z section of the same field of high- and low-phagocytic RPE cell lines #1 and #5 as indicated.

Scale bars, 10 μm.