Figure 5.

Low-Phagocytic RPE Cells Show Moderate Differences in the Visual Cycle Enzyme RPE65 and Ezrin, an Essential Constituent of RPE Microvilli

(A) Representative x-y maximal projections show ezrin, RPE65, and ZO-1 (all green) in high-phagocytic RPE line #1 and low-phagocytic RPE line #5 as indicated. Scale bar, 10 μm.

(B) X-z projections of the same fields as in (A) are shown with green protein stains as indicated, and as in (A) merged with nuclei (blue) labels.

(C) Representative immunoblot membrane of lysates from high-phagocytic RPE line #1 and low-phagocytic RPE line #5 probed sequentially for proteins as indicated.

(D) Dot plots show quantification of proteins in all four high-phagocytic lines (circles) and all four low-phagocytic lines (triangles) as indicated. Each protein was quantified compared with tubulin loading control and relative to a standard included on all gels and consisting of a mixed-cell line sample; symbols show mean of n = 3 independent samples for each line. Asterisks and lines indicate significant difference between the averages of high- and low-phagocytic RPE cell lines by Student's t test. ^∗p < 0.05.