Figure 3.

rAAV4 Allows for Genetic Manipulation of Quiescent NSCs in the Adult Hippocampus

(A) Schematic of the AAV-CMV-GFP/Cre construct. The CMV promoter drives expression of a GFP-Cre fusion gene.

(B) AAV4-CMV-Cre was injected into adult Ai9 mice, which harbor a lox-STOP-lox-tdTomato allele in the ROSA locus. Cre-mediated recombination in these mice leads to constitutive cellular expression of tdTomato.

(C) Co-localization of GFP/Cre and tdTomato 3 days after rAAV4-CMV-GFP/Cre injection into the Ai9 mice.

(D) Quantification of the percentage of tdTom⁺ rNSCs in the SGZ at 3 dpi (n = 3 mice).

(E and F) tdTom⁺ rNSCs expressed NSC markers GFAP (E) and NESTIN (F). Note the co-localization of GFAP or NESTIN in their radial processes (arrowheads).

(G) rAAV4-CMV-GFP/Cre was injected into the DG of MTMG mice.

(H) mGFP⁺ rNSCs are labeled throughout the DG at 3 dpi.

Scale bars, 20 μm. Values represent mean ± SD. See also Figure S4.