Figure 5.

rAAV4-Transduced Quiescent NSCs Exhibit Normal Electrophysiological Properties

(A) Live differential interference contrast (DIC) and fluorescence image from whole-cell electrophysiology recording showing the labeled rAAV4-CBA-tdTom cells in the DG of the Nestin-GFP. Also visible is the patch electrode, indicated by an asterisk. Scale bar, 20 μm.

(B and C) Passive electrical properties of tdTom⁺ cells characteristic of NSCs. (B) Top: overlaid series of membrane potential changes due to a series of whole-cell current injections (bottom). (C) Top: overlaid holding currents in response to a series of hyperpolarizing or depolarizing voltage steps (bottom).

(D–F) Overlapping distributions of current/voltage relationships in several tdTom⁺ and tdTom⁻ Nestin-GFP-labeled cells showing linear responses. Electrophysiological determinations of (E) resting membrane potentials and (F) membrane resistances were unchanged in rAAV4-transduced cells (n = 6 cells from 3 mice for each group).

(G) Sample DIC and fluorescent image during a whole-cell recording from a double-positive radial NSC in current-clamp mode. The tip of the recording electrode is labeled with an asterisk next to the recorded cell (yellow central cell). Scale bar, 20 μm.

(H) Large depolarizing response of the rAAV4-labeled NSC in (G) following spike application of 50 mM glutamate in the presence of 50 μM bicuculline.

Values represent mean ± SEM.