Table I.
Lipid composition of YA5512 and transformants of YA5512 made with the expression vectors pKK233-2 and pKK-pgsA
Strain | Lipid
|
||
---|---|---|---|
PE | PG | CL | |
mol% | |||
YA5512 | 96 ± 0.2 | 2 ± 0.5 | 2 ± 0.5 |
YA5512/pKK233-2 | 96 ± 1.0 | 2 ± 0.5 | 2 ± 1.0 |
YA5512/pKK-pgsA | 50 ± 2.0 | 41 ± 3.0 | 9 ± 1.0 |
Lipid classes separated on TLC plates were subjected to methanolysis, and the resultant fatty acid methylesters were analyzed by gas chromatography. Each value represents the average and sd from three independent experiments.