Table IV.
Three parameters of photosynthetic activities were measured in thylakoid membranes isolated from the wild type and the pgsA mutant of Synechocystis sp. PCC6803, which were grown in the presence or in the absence of PG
| Strain | PG | Photosynthetic Activity
|
||
|---|---|---|---|---|
| H2O → MV | H2O → pBQ | DADH2 → MV | ||
| μmol O2 mg Chl−1 h−1 | ||||
| Wild type | +a | 210 ± 15 | 250 ± 20 | 810 ± 40 |
| −b | 220 ± 20 | 240 ± 20 | 830 ± 45 | |
| Mutant | + | 190 ± 10 | 235 ± 20 | 845 ± 45 |
| − | 105 ± 10 | 0 | 770 ± 40 | |
Thylakoid membranes were treated for 5 min in darkness and photosynthetic activities were measured. The temperature of measurements was 30°C. Each value represents the average and sd from three independent measurements.
a
Thylakoid membranes were isolated from the cells grown in the presence of 20 μm PG.
b
Cells grown in the presence of PG were transferred to medium without PG, incubated for 6 d, and then thylakoid membranes were isolated.