Abstract
The polymerase chain reaction (PCR) was employed to detect human papillomavirus (HPV) 16 and 18 in cytological samples from the uterine cervix and in urine samples from the male consorts. HPV 16 was detected in 2 (25%)of 8 males whose wives were positive for HPV 16, while it was detected in only one (7%) of 14 consorts whose wives were negative for HPV 16 and 18. This is the first report of the detection of HPV 16 in urine. Viral detection in urine samples by the PCR method is a non‐invasive, convenient and useful tool for large‐scale epidemiologic studies and investigations of the mechanism of virus transmission.
Keywords: Human papillomavirus, Sexual partner, Polymerase chain reaction, Cervical cancer, Sexually transmitted disease
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REFERENCES
- 1. ) Harris , R. W. C. , Brinton , L. A. , Cowdell , R. H. , Skegg , D. C. , Smith , P. G. , Vessey , M. P. and Doll , R.Characteristics of women with dysplasia or carcinoma in situ of the cervix uteri . Br. J. Cancer , 42 , 359 – 369 ( 1980. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2. ) Zur Hausen , H. , Papillomavirus in anogenital cancer as a model to understand the role of viruses in human cancer ,. Cancer Res. , 49 , 4677 – 4681 ( 1987. ). [PubMed] [Google Scholar]
- 3. ) Yoshikawa , H. , Matsukura , T. , Yamamoto , E. , Kawana , T. , Mizuno , M. and Yoshiike , K.Occurrence of human papillomavirus types 16 and 18 DNA in cervical carcinomas from Japan: age of patients and histological type of carcinomas . Jpn. J. Cancer Res. , 76 , 667 – 671 ( 1985. ). [PubMed] [Google Scholar]
- 4. ) Saito , J. , Yutsudo , M. , Inoue , M. , Ueda , G. , Tanizawa , O. and Hakura , A.New human papillomavirus sequences in female genital tumors from Japanese patients . Jpn. J. Cancer Res. , 78 , 1081 – 1087 ( 1987. ). [PubMed] [Google Scholar]
- 5. ) Campion , M. J. , Singer , A. and Clarkson , P. K.Increased risk of cervical neoplasia in consorts of men with penile condylomata acuminata . Lancet , i , 943 – 946 ( 1985. ). [DOI] [PubMed] [Google Scholar]
- 6. ) Singer , A. , Reid , B. L. and Coppleson , M.The role of the high risk male in the aetiology of cervical cancer: a correlation of epidemiology and molecular biology . Am. J. Obstet. Gynecol , 126 , 110 – 116 ( 1976. ). [DOI] [PubMed] [Google Scholar]
- 7. ) Saiki , R. K. , Scharf , S. , Faloona , F. , Mullis , K. B. , Horn , G. T. , Erlich , H. A. and Arnheim , N.Enzymatic amplification of beta‐globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia . Science , 230 , 1350 – 1354 ( 1985. ). [DOI] [PubMed] [Google Scholar]
- 8. ) Nakazawa , A. , Inoue , M. , Saito , J. , Sasagawa , T. , Ueda , G. and Tanizawa , O.Detection of human papillomavirus types 16 and 18 in the exfoliated cervical cells using the polymerase chain reaction . Int. J. Obstet. Gynecol , ( 1991. ), in press . [DOI] [PubMed] [Google Scholar]
- 9. ) Melchers , W. J. G. , Schift , R. , Stolz , E. , Lindeman , J. and Quint , W. G.V. Human papillomavirus detection in urine samples from male patients by the polymerase chain reaction . J. Clin. Microbiol , 27 , 1711 – 1714 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10. ) Shibata , D. K. , Arnheim , N. and Martin , W. J.Detection of human papillomavirus in paraffin‐embedded tissue using the polymerase reaction . J. Exp. Med. , 167 , 225 – 230 ( 1988. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11. ) Schneider , A. , Sawada , E. , Gissmann , L. and Shah , K.Human papillomaviruses in women with a history of abnormal Papanicolaou smears and in their male partners . Obstet. Gynecol , 69 , 554 – 562 ( 1987. ). [PubMed] [Google Scholar]
- 12. ) Ritter , D. B. , Kadish , A. S‐ , Vermund , S. H. , Romney , S. L. , Villari , D. and Burk , R. D.Detection of human papillomavirus deoxyribonucleic acid in exfoliated cervicovaginal cells as a predictor of cervical neoplasia in a high‐risk population . Am. J. Obstet. Gynecol , 159 , 1517 – 1525 ( 1988. ). [DOI] [PubMed] [Google Scholar]
- 13. ) Morris , B. J. , Flanagan , J. L. , Mckinnon , K. J. and Nightingale , B. N.Papillomavirus screening of cervical lavages by polymerase chain reaction . Lancet , ii , 1368 – 1371 ( 1988. ). [DOI] [PubMed] [Google Scholar]
- 14. ) Young , L. S. , Bevan , I. S. , Johnson , M. A. , Blomfield , P. I. , Bromidge , T. , Maitland , N. J. and Woodman , C. B. J.The polymerase chain reaction; a new epidemiologic tool for investigating cervical human papillornavirus infection . Br. Med. J. , 298 , 14 – 18 ( 1989. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15. ) Shimada , M. , Fukushima , M. , Mukai , H. , Kato , I. , Nishikawa , A. and Fujinaga , K.Amplification and specific detection of transforming gene region of human papillomavirus 16, 18 and 33 in cervical carcinoma by means of the polymerase chain reaction . Jpn. J. Cancer Res. , 81 , 1 – 5 ( 1990. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16. ) Yokota , H. , Yoshikawa , H. , Shiromizu , K. , Kawana , T. and Mizuno , M , Detection of human papillomavirus types 6/11, 16 and 18 in exfoliated cells from the uterine cervices of Japanese women with and without lesions . Jpn. J. Cancer Res. , 81 , 896 – 901 ( 1990. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 17. ) Nishikawa , A. , Fukushima , M. , Shimada , M. , Yamakawa , Y. , Shimano , S. , Kato , I. , and Fujinaga , K.Relatively low prevalence of human papillomavirus 16, 18 and 33 DNA in the normal cervices of Japanese women shown by polymerase chain reaction . Jpn. J. Cancer Res. , 82 , 532 – 538 ( 1991. ). [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18. ) Burmer , G. C. , Parker , J. D. , Bates , J. , East , K. and Kulander , B. G.Comparative analysis of human papillomavirus detection by polymerase chain reaction and vira‐pap/viratype kits . Am. J. Clin. Pathol , 94 , 554 – 560 ( 1990. ). [DOI] [PubMed] [Google Scholar]