Figure 2.

BPTF Is Essential for the Maintenance and Reconstitution Function of HSCs in a Cell-Autonomous Manner

(A and B) Summary (A) and representative colony (B; scale bar, 1 mm) in colony-forming unit assays with 300 of the Bptf^f/f or Bptf^cKO (f/f; cre) LSK cells sorted 7 days after cre induction (n = 3 independent experiments; ^∗p < 0.05; ^∗∗p < 0.01; see also Figure S1C).

(C) Outline of competitive reconstitution assay via BMT.

(D) Percentage of donor-derived CD45.2⁺ cells from Bptf^cKO (blue; n = 8 mice) and control mice, either Bptf^f/f (red; n = 8) or Bptf^-/w (green; n = 6), in peripheral blood of recipients at the indicated time points. Error bars denote SE.

(E) FACS of donor-derived CD45.2⁺ cells, either from Bptf^f/f or Bptf^cKO mice, in peripheral blood 5 weeks after cre induction.

(F–H) Summary (F and G; n = 2 mice at each time point) and FACS (H) of donor-derived CD45.2⁺ cells, either from control (Bptf^f/f) or Bptf^cKO mice, in the BM LSK and LT-HSC populations 8 weeks after cre induction (see also Figure S1D).

(I and J) Percentage (I; n = 4 mice) and FACS (J) of donor-derived CD45.2⁺ cells from Bptf^f/f or Bptf^cKO mice in the indicated BM populations 8 weeks after cre induction (see also Figures S1E and S1F).