Figure 3.

HuSC Isolation from Stored Muscle

(A) HuSCs were isolated as previously described from resected adult muscle either immediately after resection or after a storage period of 1 or 4 days in 30% FBS at 4°C. Representative flow-cytometry profiles of HuSC isolation after each condition are shown (n = 3 biological replicates). Cells gated are outlined in black within each plot. The percentage of events in each gating step is shown in each plot.

(B) Bar graph depicting the average number of HuSCs isolated per gram of muscle on each day processed. No statistically significant difference among the three groups (n = 3 biological replicates).

(C) Bar graphs demonstrating the average percentage of HuSCs adhering onto Terasaki wells after isolation and seeding. There was no statistically significant difference among the three groups (n = 3 biological replicates).

(D) Bar graph showing the number of human myofibers engrafted in each mouse TA after xenotransplantation with 2,000 HuSCs into NSG TA muscles with cells isolated on day 0 (n = 4), day 1 (n = 4), or day 4 (n = 3) after biopsy (n values denote individual mice). There was no significant difference in the average engraftment of each condition.

(E) Representative images of human myofiber engraftment after xenotransplantation (n = 3 biological replicates). Scale bar, 100 μm.

Data presented as mean ± SEM. See also Figure S4.