Table 1.
qRT-PCR Analysis of Genes that Were Up- or Downregulated in Cultured dKO Satellite Cells
| Growth Medium |
Differentiation Medium |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Upregulated Genesa |
Downregulated Genesb |
Upregulated Genes |
Downregulated Genes |
||||||||
| Genec | Fold Changed (dKO/dHet) | Celle Type | Gene | Fold Change (dHet/dKO) | Cell Type | Gene | Fold Change (dKO/dHet) | Cell Type | Gene | Fold Change (dHet/dKO) | Cell Type |
| Anpep (Cd13) | 5.0 | many | Cdh15 (M-Cad) | NA | SC SkM | Bmp4 | NA | many | Cd44 | 7.1 | many |
| Bmp4 | NA | many | Cxcr4 | NA | S/P | Cebpa | 11.0 | A | Cdh15 (M-Cad) | NA | SC SkM |
| Cd34 | NA | S/P SC | Mef2c | NA | SkM | Cebpb | NA | A | Cxcr4 | NA | S/P |
| Cebpa | 4.3 | A | Met (c-Met) | 2.1 | SC | Col1a1 | 46.0 | F/M | Id1 | 6.5 | many |
| Cebpb | NA | A | Mstn | NA | SkM | Fabp4 (Ap2) | NA | A | Id2 | NA | many |
| Col1a1 | 18.3 | F/M | Myh4 | NA | SkM | Il4ra | 2.5 | many | Itga7 | 10.8 | SC |
| Fabp4 (Ap2) | NA | A | Myh7 | NA | SkM | Lpl | 6.5 | A | Lbr | 3.2 | many |
| Grem1 | 91.4 | many | Myod1 | NA | SkM | Ly6a (Sca1) | 43.5 | S/P | Mef2c | NA | SkM |
| Il4ra | 16.5 | many | Myog | NA | SkM | Pdgfra | NA | F/M | Met (c-Met) | NA | SC |
| Klf4 | 5.2 | many | Peg3 (Pw1) | 16.8 | S/P | Pdgfrb | NA | F/M | Mstn | NA | SkM |
| Ly6a (Sca1) | 125.8 | S/P | Tagln (Sm22a) | 9.2 | SM | Myh7 | NA | SkM | |||
| Numb | 3.0 | many | Twist1 | NA | many | Myod1 | NA | SkM | |||
| Pdgfra | NA | F/M | Myog | 36.3 | SkM | ||||||
| Pdgfrb | 19.2 | F/M | Peg3 (Pw1) | 21.1 | S/P | ||||||
| Pparg | 5.1 | A | |||||||||
| Sdc4 | 3.9 | SC | |||||||||
| Tagln (Sm22a) | 3.7 | SM | |||||||||
| Thy1 | NA | S/P | |||||||||
| Twist1 | NA | many | |||||||||
Genes upregulated in dKO cells compared with dHet cells.
Genes downregulated in dKO cells compared with dHet cells.
Only genes showing significant changes in gene expression (p < 0.05), or whose expression was detected in only one genotype, are shown. All genes assayed, with corresponding ΔCt values, are listed in Table S1.
Gene expression was quantified by qRT-PCR using a custom TaqMan low-density array. Fold changes were calculated using Bmpr2 as the reference gene, as it yielded highly consistent Ct values between genotypes. NA, fold change could not be calculated for genes expressed in cells of only one genotype (genes with Ct values ≤30 were considered to be expressed).
Cell types are listed for genes whose expression is cell type specific or restricted, or which are expressed by cells of a specific functional class. Satellite cells are listed for genes/proteins that are commonly used for their identification or isolation, even if more broadly expressed. A, adipogenic; F/M, fibroblast or mesenchyme; SC, satellite cell; SkM, skeletal muscle; SM, smooth muscle; S/P, stem/progenitor cell; many, genes that are broadly expressed. See also Table S1.