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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Pain. 2017 Apr 27;18(9):1046–1059. doi: 10.1016/j.jpain.2017.04.001

Figure 5.

Figure 5

Morphine tolerance correlated with Oprm1 methylation and silenced expression. (A) Relative expression and (B) methylation value for each of the treatment groups (6 mice per group) are shown. Oprm1 expression was higher in DRG from Ad-OPRM1 mice than in the Ad-GFP mice. The Ad-GFP morphine group had significantly reduced Oprm1 expression compared with the control Ad-GFP vehicle group (P = .033, 1-way ANOVA, Holm-Sidak post hoc test). Accordingly, the Ad-GFP morphine group was hypermethylated at 3 separate CpG sites in the mouse Oprm1 promoter, compared with the control Ad-GFP vehicle groups (P = .005, 2-way ANOVA, Holm-Sidak post hoc test). Abbreviations: SEM, standard error of the mean; Actb, mouse β-actin; TSS, transcription start site.