(a–b) Cytokines in mLNs after a single sensitization (a) or two sensitizations (b) with 100 μg OVA and 1.25 μg FLA. (c) T cell proliferation and cytokine production in co-cultures of OT-II T cells mixed with the indicated APC(s) sorted from lungs of mice 6h after sensitization with OVA/FLA. (d) Intracellular staining for IL-17 and type 2 cytokines in CD4+ T cells of mice sensitized with OVA/FLA op on days 0 and 7 and harvested on day 10. Lung cells were stimulated with PMA and ionomycin prior to staining. Data shown are (a) representative of 2 experiments with 8–10 mice per group; (b) from a single experiment, representative of two, 4–5 mice per group; (c) representative of a single experiment performed with duplicate samples; and (d) from a single experiment with 3–4 mice per genotype. * p < 0.05, ** p < 0.01, ***p < 0.001, ****p < 0.0001.